> There will be a workshop offered this summer on
> basic laboratory techniques
> for arthropod molecular systematics. See below:
>
> Laboratory workshop on the generation of DNA
> sequence data sets for
> molecular systematics projects, emphasizing the use
> of protein-coding
> nuclear genes. Open to 10 scientists (graduate level
> and beyond),
> particularly those affiliated with AToL and
> arthropod systematics
> generally. Many of the techniques to be covered in
> the workshop have been
> described in
> www.umbi.umd.edu/users/jcrlab/PCR_primers.pdf. The
> workshop is
> designed for those wishing to have hands-on exposure
> to these techniques in
> an instructional environment.
>
> When: June 5 - 9, 2006 (Monday - Friday noon)
> Where: University of Maryland campus (College Park,
> Maryland, USA)
> Instructors: Jerry Regier
> (www.umbi.umd.edu/~cbr/regier.html)
> Akito
>
Kawahara(www.entm.umd.edu/People/gradstudents.html#Akito)
>
> Cost: There is no registration or user fee. In
> addition, shared-room,
> near-campus housing will be provided at no cost.
> (Funded through the
> AToL/NSF award no. EF-0531626: Phylogeny of
> Lepidoptera: A Genomics-
> inspired, Community Collaboration; www.leptree.net)
>
> Workshop outline:
>
> Monday: Specimen collecting, storage, and inventory;
> nucleic acid
> extraction; PCR primer design
>
> Tuesday: Reverse-transcription Polymerase Chain
> Reaction; thermocycler
> conditions; analytical gel electrophoresis
>
> Wednesday: DNA extraction from agarose; quick
> methods for PCR clean-up;
> preparative gel electrophoresis and band isolation
>
> Thursday: Nested- and M13-PCR reamplifications;
> quantification of template;
> visit to core DNA sequencing facility
>
> Friday (a.m. only): Editing and assembling sequence
> chromatograms;
> interpreting chromatograms that are difficult to
> read; assessing problems
> with the data set
>
> How to apply: Send to Jerry Regier (regier@...)
> a CV and a description
> of why you wish to attend the workshop, including
> the systematics project
> on which you are currently engaged. Applications
> should be received by
> April 21, 2006.
>
> Further questions: Contact Jerry Regier at
> 301-405-7679 or regier@...
>






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